Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= gi|GMS|NC_004545|306612..306845..+| 306612..306845..+ [77 aas]
Database:
| Sequences producing significant alignments: | Score (bits) | E value |
|---|---|---|
| gnl|CDD|26268 pfam04551, GcpE, GcpE protein. In a variety of organisms,... | 93.0 | 7e-21 |
>gnl|CDD|26268 pfam04551, GcpE, GcpE protein. In a variety of organisms, including plants and several eubacteria, isoprenoids are synthesised by the mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Although different enzymes of this pathway have been described, the terminal biosynthetic steps of the MEP pathway have not been fully elucidated. GcpE gene of Escherichia coli is involved in this pathway..
Score = 93.0 bits (231), Expect = 7e-21
Identities = 38/67 (56%), Positives = 52/67 (77%)
Query: 2 LGTTESEGFRNGTVKSSIGIGLILLEGVGDTIRVLLAFDPVKEVKIGFDILYTLNMKSRG 61
LG TE+ G +GTVKS++ +G +L EG+GDTIRV L DPV+E+K+G DIL +L ++SRG
Sbjct: 197 LGVTEAGGLFSGTVKSAVALGALLSEGIGDTIRVSLTADPVEEIKVGRDILKSLGLRSRG 256
Query: 62 VNFIAYP 68
VN I+ P
Sbjct: 257 VNIISCP 263
| Parameter | Value |
|---|---|
| allowgaps | yes |
| expect | 1.0e-02 |
| gapext | 100 |
| gapopen | 1100 |
| matrix | BLOSUM62 |
| Statistic | Value |
|---|---|
| A | 40 |
| Hits_to_DB | 674,774 |
| S1 | 4100 |
| S1_bits | 1910.6 |
| S2 | 84 |
| S2_bits | 36.6 |
| T | 11 |
| X1 | 1600 |
| X1_bits | 744.5 |
| X2 | 3800 |
| X2_bits | 1463.8 |
| X3 | 6400 |
| X3_bits | 2465.3 |
| dbentries | 0 |
| effective_hsplength | 46 |
| effectivedblength | 2336118 |
| effectivespace | 72419658 |
| effectivespaceused | 72419658 |
| entropy_gapped | 0.140 |
| kappa_gapped | 0.0515 |
| lambda_gapped | 0.267 |
| num_extensions | 40625 |
| num_successful_extensions | 31 |
| number_of_hsps_better_than_expect_value_cutoff_without_gapping | 1 |
| querylength | 31 |
| seqs_better_than_cutoff | 1 |